 {"id":76,"date":"2015-03-27T13:10:37","date_gmt":"2015-03-27T13:10:37","guid":{"rendered":"http:\/\/www.international-bio-consulting.com\/?page_id=76"},"modified":"2018-04-03T07:17:18","modified_gmt":"2018-04-03T07:17:18","slug":"research-expertise","status":"publish","type":"page","link":"https:\/\/www.international-bio-consulting.com\/?page_id=76","title":{"rendered":"Research Expertise"},"content":{"rendered":"<h3>Anna T. Salek,\u00a0 Ph.D.,\u00a0 D.Sc. (Dr. habil.)<\/h3>\n<p><strong>TOPICS\u00a0 OF\u00a0 EXPERTISE\u00a0 AND\u00a0 RESEARCH<\/strong><br \/>\n<strong>I.\u00a0Hands-on experience in food biotechnology, i.e.:<\/strong><\/p>\n<ul>\n<li>in microbial physiology (e.g. optimalisation of growing and fermentation processes),<\/li>\n<li>in biochemistry of enzymes and proteins (i.e., development, recovery and purification<\/li>\n<li>of protein: analytical and preparative scale), in regulation of microbial metabolism,<\/li>\n<li>in molecular cell biology,<\/li>\n<li>in applied microbiology, especially on the improvement of bacteria and yeast strains, both in the laboratory and in industry scale (Probiotics in Functional Food),<\/li>\n<li>in Food Safety Microbiology (HACCP-System for pathogens, Risk Assessment,\u00a0Microbial Food Monitoring, International Food Standard).<\/li>\n<li>In environment (water and air): new methods of identification (biosensors).<\/li>\n<\/ul>\n<p>It included:<\/p>\n<ul>\n<li>Work on the bacteria Escherichia coli, Enterobacter aerogenes and Salmonella\u00a0typhimurium isolated from dairy products, i.e., microbiological and biochemical\u00a0diagnostic, a chemical and a physical mutagenesis of cells, regulation of bacterial\u00a0metabolism, especially work on biochemistry of \u00df-galactosidase and pyruvate\u00a0 ehydrogenase complex. Results were applied in cheese-making (see papers in\u00a0 \u201cPublication List\u201d).<\/li>\n<li>Microbiological work on the technological aspects of dairy products, optimalisation\u00a0of fermentation processes (beer &amp; alcohol beverage, mathematical modelling),\u00a0probiotics and functional food as well as work on the animal feeds (probiotics, starter\u00a0culture) (see\u00a0 4 patents and papers in \u201cPublication List\u201d).<\/li>\n<li>Genetic work on the killer protein of yeast &#8211; secretion system (especially on bakery\u00a0and wine strains), constructed by electro-transformation of dsRNA (and also co-electro-transformation with plasmid pADH) or of &#8222;Virus Like Particles&#8220; with dsRNA\u00a0 into cells (stipend of UNDP\/FAO-\u00a0 Roma and project at the University of W\u00fcrzburg,\u00a0Institute of Microbiology and Genetics, Department of Biotechnology, Germany).\u00a0See papers in \u201cPublication List\u201d.\u00a0Strains developed in ways described above were used in the food technology, in the agriculture, in the industrial fermentation processes and in the production of baker&#8217;s yeast as well as for alcoholic beverages, during the course of collaborative work (consulting and application of new strains) with the fermentation industry in Poland (the former government-owned &#8222;Polmos&#8220; companies and the Institute of Biotechnology in Warsaw, Poland) from 1980 up to now.<\/li>\n<li>Work on the cytoskeleton of yeast cells (cell biology): a new technique that was\u00a0developed and used within my research was enucleation, i.e., technique for the\u00a0 removal of nucleus from cell\/protoplasts of Saccharomyces cerevisiae (including\u00a0 fluorescence microscopic analyses, micrography). It was used for the construction of\u00a0cybrids (by electro-fusion technique), i.e., new yeast hybrids possessing mixed\u00a0cytoplasms but only one nucleus after enucleation; such cybrids could be designed\u00a0to obtain new yeast industry strains (for food biotechnology) with particular abilities,\u00a0such as that of secretion of certain molecules (and also for research on RNA or DNA-viruses, i.e., VLPs). Work at the University of W\u00fcrzburg, Institute of Genetic and\u00a0Microbiology, Germany (see papers in \u201cPublication List\u201d).<\/li>\n<li>Biochemical work on the purification of killer yeast protein (glycoproteins),\u00a0i.e., gel filtration, ion exchange chromatography, isoelectrofocusing (analytical and\u00a0preparative-scale), as well as PAGE (native or with SDS, IPG\/gradient pH or Gradient\u00a0PA), 2-D(2-dimensional electrophoresis, i.e. IPG\/2-D-SDS PAGE), an ultrafiltration\u00a0and concentration (&#8222;Amicon&#8220; systems and freeze-drying\/lyophilisation techniques) of\u00a0proteins and also different methods for dialysis process, etc. Purified killer Protein\u00a0could be used as biological control for pathogen\u00a0 infections. Work at the Technical\u00a0University of Munich-Weihenstephan, Germany (see papers in \u201cPublication List\u201d);<\/li>\n<li>Work on the PCR (molecular biology) (Inter-LINE-PCR as well as on the RAPD-PCR\u00a0and AP-PCR) and on the separation of chromosomes (by PFGE, pulse field gel\u00a0electrophoresis) for the characterisation of medical and industrial: brewing, wine and\u00a0bakery strains (at the GSF &#8211; National Research Centrum for Environment and Health,\u00a0GmbH in Munich, and at the Technical University of Munich-Weihenstephan, Institute\u00a0of Technical Microbiology and Technology of Brewing, Germany, respectively).\u00a0Results were used for development of automated yeast identification system by the\u00a0application of PCR technique\u00a0 (see papers in \u201cPublication List\u201d). Most of my research in cell biology has been on Saccharomyces spp., but some was on Kluyveromyces spp. and especially recently &#8211; on Pichia (Hansenula) spp. (with expression of killer protein-toxin, source of dsRNA and dsDNA plasmids), Candida spp. and Fusarium spp., which exist also in food (especially in wine).<\/li>\n<li>Food &amp; Environment Safety Microbiology and Health Protection System: Food Safety,\u00a0 HACCP, IFS, GMO, Functional Food, Nutraceuticals.\u00a0 Routine &amp; research work on biochemical, microbiological and immunological\u00a0identification of pathogenic microorganisms in drink and waste water (bacteria,\u00a0yeasts and fungi, i.e. Cryptosporidium parvum, Campylobacter, Escherichia coliserotype O157:H7 (EHEC), Legionella pneumophila, Candida albicans, Aspergillus\u00a0fumigatus, Fusariums, etc.) as well as in food products (Salmonella, Listeria,\u00a0Escherichia coli serotype O157:H7 (EHEC) and Campylobacter jejuni).\u00a0Work for analytical laboratory of food products and environment, using Food Safety\u00a0and Protection System, i.e. HACCP (Hazard Analysis Critical Control Point\u00a0 and\u00a0 predictive microbiology), microbiological monitoring and modern, rapid &amp; safety\u00a0 methods (e.g. immunology, genetic, bio-sensors). For this case see paper (Salek A.:<br \/>\nA modern microbiology methods in food monitoring, HACCP systems and in\u00a0 diagnostics. The Journal of Food Industry. 2004).<\/li>\n<\/ul>\n<p><strong>II.\u00a0Experience in teaching of food biotechnology<\/strong><\/p>\n<p>I have had several years experience in the teaching of general microbiology, food biotechnology (aspects of molecular biology), as well as food technology (functional food, especially dairy and yeast fermentation) at the University of Warsaw and also in industry (see enclosed CV) as well as at the University W\u00fcrzburg and Munich (Germany).<br \/>\nI had also many congresses, seminars, written monographs and summer-schools for students and industrial microbiologists on the microbiological topics, i.e. applications of molecular biology in food technology, about transgenic and new microorganisms, like Legionella pneumophila, Cryptosporidium parvum or about biological control of pathogen infections or biofilm in practice (water in different systems).<\/p>\n<p><strong>III.\u00a0Hands-on experience in biotechnology of pharmaceutical, i.e.:<\/strong><\/p>\n<ul>\n<li>in molecular cell biology of yeast and bacteria,<\/li>\n<li>in biochemistry of yeast proteins (i.e. development, recovery and purification of recombinant proteins: analytical and preparative scale), in regulation of yeast metabolism,<\/li>\n<li>in yeast and bacteria physiology (optimalisation of growing and Fermentation\u00a0processes),<\/li>\n<li>in applied of killer-yeast-proteins (drug-glycoprotein), especially in the industry scale,<\/li>\n<li>vet. medicine safety \u2013 microbiological control of pharmaceutical products,\u00a0according to Ph. Eur. 2001.<\/li>\n<\/ul>\n<p>It included:<\/p>\n<ul>\n<li>Regulatory Affairs (MRLs) work on the pharmacology (pharmacodynamics and\u00a0pharmacokinetics: absorption, distribution, metabolism, excretion) as well as\u00a0toxicology (toxicity, mutagenicity, teratogenicity, carcinogenicity) of yeast killerprotein using mammalian cells culture (killer toxin as a new antimicrobial agents for\u00a0oral application, against pathogenic bacteria, yeasts and fungi, infected domestic\u00a0animals); work at the firm \u201eInropharm\u201c GmbH &amp; Co. and Technical University of\u00a0Munich, Germany (see papers in \u201cPublications List\u201d).<\/li>\n<li>Registration of new antimicrobial agent and veterinary medicine in EU (Report on the Safety and Residue File) for EU\u00a0 in Brussels\/London (EMEA), work as Regulatory Affair Scientist for \u201eInropharm\u201c GmbH &amp; Co., Germany (3 Monographs) and for German Ministry of Health in Berlin (2 Monographs).<\/li>\n<li>Work at the pharmaceutical firm for a production and validation of methods for\u00a0 chemical analysis of veterinary medicines (\u201eInropharm\u201c GmbH &amp; Co., Germany).<\/li>\n<li>Sterility (Sterilit\u00e4t) activity of preservative (Konservierungsstoffe) control of\u00a0veterinary products (especially medicines for injection), according to book Pharm.\u00a0Eur. 2001, 2002.<\/li>\n<li>General hygiene control in pharmacy industry, according to GMP\/GHP requirements.<\/li>\n<\/ul>\n<p><strong>IV.\u00a0Experience as manager of food biotechnology and as pharmaceutical and environmental\u00a0\u00a0laboratory<\/strong><\/p>\n<p>Over 10 years I was the head of microbiology laboratory at the Agricultural University of Warsaw as well as at the Institute of Biotechnology (Warsaw, Poland) and while there, I managed several research projects in biotechnology area. Next, over 28 years I was the head of microbiology laboratory: at the pharmaceutical firm, environmental firm and also at the Microbiology Institute (Germany) as head of Food &amp; Environment &amp; Veterinary &amp; Feed Microbiology, Biotechnology (incl. veterinary with Bovine Spongiforme Encephalopathy, BSE) and Immunology Laboratory. Last 12 years I have been manager Microbiology &amp; Chemistry Labor for Analytic of Water &amp; Environment (incl. fungi diagnostic). It was work on Legionella pneumophila problematic (Routine and Research with patent About new method for rapid identification biofim-bacteria, incl. Legionella pneumophila).<\/p>\n<p><strong>V.\u00a0Future research in food &amp; environment &amp; pharmaceutical biotechnology<\/strong><\/p>\n<p>As a microbiologist with hands-on experience in the application of novel techniques to cell engineering, I have worked (patent) on the use of naturally-produced peptides or proteins from yeasts for the therapeutic treatment of infected animals, i.e. for diseases caused by pathogenic bacteria, yeasts and fungi (use of killer yeast toxins for biological control).<\/p>\n<p>I have established strong evidence that some yeast toxins are good anti-microbial agents (like probiotics) and can be effective, as pre-therapeutics in the treatment of antibiotic-resistant intestinal (E.coli, Enterobacter aerogenes, Salmonella typhimurium, Enterococcusspp., etc.) and other infections (e.g. Styphylococcus aureus, Pasteurella haemolytica, Candida albicans, Acremonium or Aspergillus fumigatus, etc.) in several domestic animals (see papers in \u201cPublications List\u201d).<\/p>\n<p>It could be a big interest to continue farther this topic. Future research in biotechnology they are modern &amp; rapid &amp; safety instrumental as well as electrochemical methods, connected with experiences of follow sciences:<\/p>\n<ul style=\"list-style-type: square;\">\n<li>\uf0fc molecular biology<\/li>\n<li>\uf0fc immunology<\/li>\n<li>\uf0fc biophysics and<\/li>\n<li>\uf0fc chemie<\/li>\n<\/ul>\n<p>See paper (Salek A.: A modern microbiology methods in food monitoring, HACCP systems and in diagnostics. The Journal of Food Industry. 2004).<\/p>\n<p><strong>VI.\u00a0Experience in accreditation of biological laboratory according to ISO 17025:2008 norm<\/strong><\/p>\n<p>In 1999, 2000, 2004, 2005, 2010 und in 2014 were prepared documents (QMH, SOP\u00b4s and others) for accreditation microbiological and chemical laboratory (in water and food technology<br \/>\nfield) according to ISO norm 17025:2005. Accreditation Office: DAP (now DAkkS), Berlin, Germany.<\/p>\n<p>&nbsp;<\/p>\n<style><\/style>\n","protected":false},"excerpt":{"rendered":"<p>Anna T. Salek,\u00a0 Ph.D.,\u00a0 D.Sc. (Dr. habil.) TOPICS\u00a0 OF\u00a0 EXPERTISE\u00a0 AND\u00a0 RESEARCH I.\u00a0Hands-on experience in food biotechnology, i.e.: in microbial physiology (e.g. optimalisation of growing and fermentation processes), in biochemistry of enzymes and proteins (i.e., development, recovery and purification of protein: analytical and preparative scale), in regulation of microbial metabolism, in molecular cell biology, in &#8230; <a title=\"Research Expertise\" class=\"read-more\" href=\"https:\/\/www.international-bio-consulting.com\/?page_id=76\" aria-label=\"Mehr Informationen \u00fcber Research Expertise\">Weiterlesen<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-76","page","type-page","status-publish"],"_links":{"self":[{"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=\/wp\/v2\/pages\/76","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=76"}],"version-history":[{"count":14,"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=\/wp\/v2\/pages\/76\/revisions"}],"predecessor-version":[{"id":177,"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=\/wp\/v2\/pages\/76\/revisions\/177"}],"wp:attachment":[{"href":"https:\/\/www.international-bio-consulting.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=76"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}